GB 5009.285-2022 Translated English of Chinese Standard (GB5009.285-2022): National food safety standard - Determination of Vitamin B12 in foods [Buy--download True-PDF in 3-second: https://play.google.com/books > Sign in > This BOOK > "3-dots" > Export > Save as "*.pdf"]https://www.chinesestandard.net, 2022 M08 21 - 27 pages This Standard specifies the determination method of vitamin B12 in foods. Method I of liquid chromatography applies to the determination of vitamin B12 in foods for infants and young children, milk and milk products, meat and meat products. |
Contents
Instruments and equipment | 18 |
Test procedures | 19 |
Description of the analysis results | 21 |
Precision | 22 |
Appendix A Immunoaffinity column reference verification method | 23 |
Appendix B Liquid chromatogram | 24 |
Liquid chromatography mass spectrum | 25 |
Medium preparation method | 26 |
Principle | 16 |
Common terms and phrases
100 mL volumetric accurate to 0.01 Acetonitrile adjust the pH ammonium acetate B12 in foods B12 isotope internal B12 standard stock brown reagent bottle CAS number chromatographic cobalamin concentration of vitamin conversion coefficient cyanide or sodium determination of vitamin fiber filter paper fix the volume foods for infants glass fiber filter immunoaffinity column infants and young isotope internal standard Lactobacillus light transmittance Liquid chromatography mass spectrometry mL of vitamin mL of water mL volumetric flask mL/min peak area pipette potassium cyanide potassium dihydrogen phosphate preparation method r/min sample extract sampling amount sensitivity 0.01 g sodium acetate sodium acetate buffer sodium chloride sodium cyanide sodium hydroxide sodium metabisulfite solution to dissolve solution to fix standard curve standard solution standard stock solution standard substance standard vitamin B12 standard working solution test bacteria solution to-be-tested solution transfer trifluoroacetic acid trifluoroacetic acid solution value of vitamin Vitamin B12 isotope vitamin B12 standard water to dilute